TROPLIST: Brightest Fluorescent Proteins

[Mustafa Khokha]

O.k. so as many of you new there are a number of different  
fluorescent proteins available now.
It would be useful to know which were the brightest in Xenopus for a  
lot of different studies.
So I've collected many of them and devised a simple test.  We have  
two fluorescent filter sets
here on our scope - GFP and rhodamine.  We injected 100 pg of RNA  
into 1 cell at 2 cell and then
scored them the next day (mid st 20's) and then just looked at them  
and tried to judge brightness.

I was a little surprised by the results since I had some preconceived  
notions based on Tsien's groups
recent review Nature methods Dec 2005.

For the GFP filter,
YFP appeared brightest.  Next was GFP which was a close second.  Both  
of these were brighter than
Venus.  And all of these were much brighter than mCitrine.

For the Rhod filter
tdTomato was the brightest clearly.  Then mCherry and RFP were  
similar and bright.  They were all
much brighter than mStrawberry.  mOrange was weak.

I've tried this twice now, gotten identical results, and was a bit  
surprised.  Has anyone else tried something similar?  Since my results
were different than what you might expect after reading Tsien's  
review, I am wondering if I just had some fatal
flaw in my experiment.  Obviously there is a subjective component.

Mustafa



Mustafa K. Khokha
Assistant Professor, Pediatrics & Genetics
Yale University School of Medicine
333 Cedar Street/LCI 305
PO Box 208064						
New Haven CT 06520-8064			
Ph: (203) 785-4651
Fax: (203) 785-5833

For Courier Shipments
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