TROPLIST: Brightest Fluorescent Proteins

[Takuya Nakayama]

Hi Mustafa:

Quick question: Do those all have the same 5' and 3'-UTRs?  If they  
have different UTRs one another, stability of each mRNA (and also  
translation efficiency?) may be different.  Just curious.

Takuya


On Aug 2, 2007, at 5:41 PM, Mustafa Khokha wrote:

> O.k. so as many of you new there are a number of different  
> fluorescent proteins available now.
> It would be useful to know which were the brightest in Xenopus for  
> a lot of different studies.
> So I've collected many of them and devised a simple test.  We have  
> two fluorescent filter sets
> here on our scope - GFP and rhodamine.  We injected 100 pg of RNA  
> into 1 cell at 2 cell and then
> scored them the next day (mid st 20's) and then just looked at them  
> and tried to judge brightness.
>
> I was a little surprised by the results since I had some  
> preconceived notions based on Tsien's groups
> recent review Nature methods Dec 2005.
>
> For the GFP filter,
> YFP appeared brightest.  Next was GFP which was a close second.   
> Both of these were brighter than
> Venus.  And all of these were much brighter than mCitrine.
>
> For the Rhod filter
> tdTomato was the brightest clearly.  Then mCherry and RFP were  
> similar and bright.  They were all
> much brighter than mStrawberry.  mOrange was weak.
>
> I've tried this twice now, gotten identical results, and was a bit  
> surprised.  Has anyone else tried something similar?  Since my results
> were different than what you might expect after reading Tsien's  
> review, I am wondering if I just had some fatal
> flaw in my experiment.  Obviously there is a subjective component.
>
> Mustafa
>
>
>
> Mustafa K. Khokha
> Assistant Professor, Pediatrics & Genetics
> Yale University School of Medicine
> 333 Cedar Street/LCI 305
> PO Box 208064						
> New Haven CT 06520-8064			
> Ph: (203) 785-4651
> Fax: (203) 785-5833
>
> For Courier Shipments
> 333 Cedar Street/FMP 425
> New Haven CT 06520-8064			
>
>
>

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