TROPLIST: Froglet mortality

[Peterson.Laura@epamail.epa.gov]

Dear Troplisters, 

We are experiencing excessive froglet mortality in our trop culture, 
occurring approximately from stage 63 to 2 weeks post-metamorphosis. Has 
anyone seen a similar case? 

I'm suspicious of a fungal infection. We house X. laevis in a nearby lab 
and try to minimize contact between the species, but we have some traffic 
between the rooms (e.g., staff and occasionally tadpole food). We brought 
unaffected froglets (about 2 weeks post-MM) into the room where the 
froglets were dying and treated half with formalin and malachite green. 
The treated froglets survived, and those left untreated died. 

We use bleach and 70% ethanol to sterilize equipment, but are fearful of 
this infection spreading. I am very appreciative of any advice or 
information offered. 

Many thanks for your time, 

Laura Peterson 

Laura Peterson
Aquaculturist
Wilson Environmental c/o U.S. EPA
218-529-5188
peterson.laura@epa.gov
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