TROPLIST: post-natal lethargy

[Takuya Nakayama]

Hi Kris:

I think it's obviously something wrong.  We do not use Sigma one, so  
cannot directly compare but how many units hormone do you use?  We  
usually prime with 10 units and boost with 100 units of HCG in 0.1 ml  
for injection.

Takuya (in Grainger lab, UVA, USA)


On Feb 5, 2007, at 11:27 AM, kris vleminckx wrote:

> Dear all,
>
> we have recently noticed that upon boosting and egg-laying of  
> trops, they become very lethargic. They do not show any escape  
> responses and just float at the surface. Occasionally they may die  
> the next day. Is this a normal reaction to the hormone-boost (I  
> have not found a human volunteer to try it out), or should I  
> suspect that something slipped in our (Sigma) HCG stock and causes  
> this reaction?
>
> Thanks for your thoughts
>
> Kris
>
> --
> Kris Vleminckx, PhD
> Dept. Molecular Biomedical Research
> Unit of Developmental Biology
> VIB - Ghent University
> Technologiepark 927
> B-9052 Ghent (Zwijnaarde), Belgium
>
> Tel : +32-(0)9-33-13720 (office)/13722 (lab)
> Fax : +32-(0)9-33-13609
> E-mail : kris.vleminckx@dmbr.UGent.be
> URL: http://www.dmbr.UGent.be