TROPLIST: myc tag antibody

[Kurt Marek]

Hi all,
We are myc tagging some proteins using the pCS2-MT vector and have  
been using immunofluorescence to detect the expressed proteins. This  
seemed to work great until we tested the antibody (Abcam Ab32 9E10  
anti-myc) on wildtype embryos and saw bright nuclear staining.  
Clearly we are picking up background or endogenous c-myc. Does anyone  
have any experience with similar experiments and can you suggest an  
antibody?

Thanks,
Kurt




Kurt Marek, Ph.D.

Division of Biological Sciences, Neurobiology Section
University of California, San Diego
9500 Gilman Dr., Pacific Hall 3222B
Mail Box 0357
La Jolla, CA 92093
Ph: 858-534-2456
kmarek@ucsd.edu