TROPLIST: RNA purification for microarray analysis

[Audic Yann]

Dear colleagues,

in response to Jose Luis Mullor regarding the RNA quality for Microarray 
analysis.
We prepared Total RNA from early stages embryos (very early like oocytes 
to 128 cells laevis or tropicalis) using tri-reagent procedure. When 
total RNA was analyzed on a bioanalyzer, we would always get a small 
peak just below the 28S RNA that would mess up the RIN calculation. This 
peak was also observed by other colleague suggesting that it may be a 
characteristic of the xenopus total RNA.
Total RNA extraction were however reproducible and gave good yield when 
used for RNA labeling.

Hope this help,

best regards,
Yann

-- 
Yann Audic

Chargé de Recherche CNRS
UMR 6061-Génétique et Développement

Faculté de médecine
2, Avenue du Professeur Léon Bernard
35043 RENNES Cedex
FRANCE

tel : 33 (0)2 2323 4475
fax : 33 (0)2 2323 4478
yann.audic@univ-rennes1.fr