TROPLIST: ChIP in embryos

[Kurt Marek]

I'm planning to perform some chromatin immunoprecipitations using  
trop embryos, but we have never ventured into this territory so I'd  
like to get any advice people may have to offer. I am planning to use  
the Upstate ChIP kit.

1) Does anyone have any experience using this kit?
2) What protocol do people use to harvest the starting material prior  
to cross-linking? I'm planning to use st 22-28 embryos, but I'm  
guessing it would be similar to that used for other stages.
3) What cross-linking protocol do you use? Does it differ from  
standard protocols designed for culture cells?
4) What antibodies have you had success with?

Thanks,
Kurt





Kurt Marek, Ph.D.

Division of Biological Sciences, Neurobiology Section
University of California, San Diego
9500 Gilman Dr., Pacific Hall 3222B
Mail Box 0357
La Jolla, CA 92093
Ph: 858-534-2456
kmarek@ucsd.edu