TROPLIST: embryo dissociation, FACS

[Kurt Marek]

Hi all,
We are trying to do some FACS sorting and would ideally like to find a  
way to start with whole embryos. The problem, of course, is  
dissociating them. Has anyone tried this or have any ideas on what  
might work?

We are working with stage 20-30 embryos and have tried acetone to  
remove the skin, but this is hard to control. We've also tried various  
concentrations of collagenase and trypsin/EDTA.

Thanks,
Kurt




Kurt Marek, Ph.D.

Division of Biological Sciences, Neurobiology Section
University of California, San Diego
9500 Gilman Dr., Pacific Hall 3222B
Mail Box 0357
La Jolla, CA 92093
Ph: 858-534-2456
kmarek@ucsd.edu