TROPLIST: Nasco tropicalis infertile

[Lyle Zimmerman]

Hi Christof-
    We haven't gotten Nasco trops for a couple of years, but have had 
good experiences with them prior to that.  The two things I'd also 
consider are whether there is a strain-compatibility issue (what is 
the origin of your non-Nasco trops?) and whether any endocrine 
disruption has happened (did the Nasco testes look multilobate or 
have any very large cells, i.e. hermaphroditic?).  We think we've 
seen this in our home-bred trops from time to time, most likely from 
endocrine disruptors leaching from plastic.  Strain compatibility is 
going be a worry if your stocks or their immediate forbears were 
wild-caught (esp if the stocks haven't been karyotyped).

best wishes
Lyle



>We have had problems with Nasco tropicalis. In the last batch we 
>received from them in 2008 both males and females are infertile. 
>Females do not lay eggs at all upon priming. Male frog testis do not 
>fertilize eggs of non-Nasco origin, which however fertilize well 
>with other testis.
>
>We first thought that the animals were still to young (even though 
>they were sold as >6 months old). Now they are another 5 months 
>older an the animals are still infertile.
>Unrelated trops which are older are working OK, so it is not housing 
>conditions but definitely to do with Nasco frogs.
>
>Anybody else have recent infertility problems with Nasco tropicalis ?
>
>
>
>Prof. Christof Niehrs
>Division of Molecular Embryology
>German Cancer Research Center
>Im Neuenheimer Feld 581
>69120 Heidelberg
>Germany
>
>Tel +49-6221-42-4690
>FAX +49-6221-42-4692
>homepage: 
><http://www.dkfz.de/en/mol_embryology/index.html>http://www.dkfz.de/en/mol_embryology/index.html
>email: <mailto:niehrs@dkfz.de>niehrs@dkfz.de
>
>TROPLIST: xenopus one or nasco?
>
>Kurt Marek 
><mailto:kmarek%40ucsd.edu?Subject=TROPLIST%3A%20xenopus%20one%20or%20nasco%3F&In-Reply-To=>kmarek@ucsd.edu
>Wed Dec 20 17:51:02 EST 2006
>
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>
>Where do people order their trops from? We've been getting them from 
>Nasco because that's where we have always purchased our laevis, but 
>we are considering a switch. Nasco refused to replace a frog that 
>died a day after arrival and they refuse to guarantee the safe 
>arrival of an $1100 order due to the weather. In addition, they are 
>much more expensive than Xenopus One. Has anyone had experience with 
>Xenopus One frogs? We are in need of an 'emergency' supply of males 
>as our in-house breeding hasn't been able to keep up lately.
>
>Thanks,
>Kurt
>
>
>
>
>
>Kurt Marek, Ph.D.
>
>Division of Biological Sciences, Neurobiology Section
>University of California, San Diego
>9500 Gilman Dr., Pacific Hall 3222B
>Mail Box 0357
>La Jolla, CA 92093
>Ph: 858-534-2456
><mailto:kmarek@ucsd.edu>kmarek@ucsd.edu
>
>
>
>
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