TROPLIST: how to get firm eggs for REMI?

[Kurt Marek]

We have been using the meganuclease procedure to generate transgenics,  
but have some experiments that would benefit from REMI and are having  
some trouble getting firm eggs for nuclear transfer. Does anyone have  
any tips? We've seen some reports suggesting that having the females  
lay into high salt media is beneficial (I think this is from Mustafa),  
while others report that trop eggs are very sensitive to high salt and  
should be kept in low salt. Also, there was one report suggesting that  
the conductivity of the water that the females are raised in makes a  
difference. This hasn't been a problem for the meganuclease procedure  
since that uses fertilized eggs. Any suggestions?

Thanks
Kurt