TROPLIST: biotinylated anti-dig and/or in situs on sections?

[Lyle Zimmerman]

We're struggling to get a good in situ signal from late-40s stage 
trop tads.  We're trying various protocols for in situs on sections, 
but it's possible that our target is expressed at very low levels.

Does anyone have experience with amplification using biotinylated 
anti-dig followed by streptavidin-conjugated chromogen or variants?

Barring that, we gratefully accept other bombproof protocols for in 
situs on tadpole sections.  Wholemounts at these stages are 
problematic...

thanks
Lyle