TROPLIST: biotinylated anti-dig and/or in situs on sections?

[Ryan Kerney]

Hi Lyle,

I have tried the TSA kit, but it didn't give very good results.  
Others swear by it though. This is the normal protocol I use for  
paraplast sections. You may get better results with cryo-sections,  
although the histology isn't as good.

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While not bomb proof this protocol does work. Try a positive control,  
such as anti-type-II collagen, which is expressed in all cartilages  
during stg. 40.

Good luck,
Ryan