TROPLIST: morpholino stability

[ilblitz@uci.edu]

Dear Troplisters,

I would like to ask you if you have found that some of your morpholino
oligos might have lost their "activity" over time?  I have just determined
that a morpholino I have been keeping at -20oC for the past 5 years and
now have a reason to dig out for an experiment, no longer has the youthful
vigor it once did.  I am aware that many people heat their morpholinos to
65oC for a short time before injecting, to improve solubility, etc.  This
also failed to divert my embryos from their normal development.  So I
thought I'd send out this email to see what others in the community have
experienced.  Thanks for any comments you might have.

Sincerely,

Ira