From maxime.willems@ugent.be Wed Nov 4 03:38:35 2009 From: maxime.willems@ugent.be (maxime willems) Date: Wed, 4 Nov 2009 09:38:35 +0100 Subject: TROPLIST: protocol retrieve gfp in sections Message-ID: <7E546846-A378-45AE-BEF2-4E2C3613E216@ugent.be> Hi, I'm looking for a protocol to retrieve the gfp signal on sections (through anti-gfp antibody reactions). I've cryo embedded some but I need cellular resolution so I believe paraffin would be better to stain. I had to fix (PFA) and deminaralise the transgenic tadpoles because of the characteristics of the tissue I wanted to analyse. Thank you for the help. best regards, Maxime